Erastin愛(ài)拉斯?。ㄨF死亡激活劑）

產(chǎn)品簡(jiǎn)介：

鐵死亡（Ferroptosis）是一種鐵依賴(lài)形式的非凋亡性細胞死亡。愛(ài)拉斯?。?span>Erastin）是一種鐵死亡激活劑（誘導劑）。體外誘導鐵死亡性細胞壞死，這一效應可被鐵死亡抑制劑（比如：Ferrostatin-1、Trolox、環(huán)匹羅司乙醇胺、放線(xiàn)菌酮和β-巰基乙醇）所阻斷。HT-1080纖維肉瘤和Calu-1肺腫瘤細胞中，愛(ài)拉斯?。?span>5 μM）通過(guò)谷氨酸-胱氨酸反向轉運體（System Xc-）抑制胱氨酸攝??；以及在酶結合的熒光分析實(shí)驗中抑制谷氨酸釋放。在表達Ras和SV40小T腫瘤蛋白的細胞中選擇性誘導細胞死亡（IC50s = 1.25-5 μg/ml）。

產(chǎn)品特性：

1） 化學(xué)名：2-[1-[4-[2-(4-chlorophenoxy)acetyl]-1-piperazinyl]ethyl]-3-(2

-ethoxyphenyl)-4(3H)-quinazolinone

2） CAS NO：571203-78-6

3） 分子式：C₃₀H₃₁ClN₄O₄

4） 分子量：547.04

5） 外觀(guān)：固體

6） 純度：≥98%

7） 溶解性：溶于DMSO（≥5mg/ml，需要溫育助溶）、不溶于乙醇、不溶于水

保存條件: 

-20oC干燥保存，至少2年有效。

儲存液制備：

【溫馨提示】：請根據產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；本品在溶液狀態(tài)不穩定，建議現配現用。

產(chǎn)品使用： 【源自文獻，僅作參考】

文獻1，Liang, Z., Wu, Q., Wang, H. et al. Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling. Cell Death Discov. 8, 190 (2022). https://doi.org /10.1038/s41420-022-00975-w

體內研究（動(dòng)物模型）：

動(dòng)物模型（Animal Model）：Endometriosis mouse model

實(shí)驗方法（Assay）：Seven-to-8-week-old C57BL/6 female mice were obtained and 17-β-estradiol-3-benzoate (30μg/kg) was administered to each mouse every day for 3 days. We removed uterine horns from the donor mice and added them to saline. Endometrium was cut into 1mm2 fragments. The endometrial fragments from each uterine horn were suspended in 0.3ml saline and injected into the peritoneal cavities of recipient mice with an 18-gauge needle. At 8 days (5 days after the operation), endometrial-like lesions were established, and they were randomly divided into two groups (each group contained 12 mice).In the experimental group, each mouse received erastin (20mg/kg/day) by intraperitoneal injection over a 7-day period. In the control group, DMSO was used instead of erastin.At 15 days, the mice were sacrificed and endometriotic lesions were collected.

文獻2，Huo H, Zhou Z, Qin J, Liu W, Wang B, Gu Y. Erastin Disrupts Mitochondrial Permeability Transition Pore (mPTP) and Induces Apoptotic Death of Colorectal Cancer Cells. PLoS One. 2016 May 12;11(5):

e0154605. doi: 10.1371/journal.pone.0154605. PMID: 27171435; PMCID: PMC4865238.

體內研究（動(dòng)物模型）：

動(dòng)物模型（Animal Model）：HT-29 tumor bearing SCID mice

實(shí)驗方法（Assay）：Briefly, 2×106viable HT-29 cells in 100 μL of growth medium (per mouse) were subcutaneously inoculated, and mice bearing ~100 mm3tumors were randomly divided into three groups with 10 mice per group.Mice were treated daily with 10 or 30 mg/kg body weight of erastin (intraperitoneal injection, for 4 weeks) or vehicle control (Saline).

注意事項：

1.      針對溶解性比較差的化合物，可通過(guò)37℃孵育以及超聲的方式來(lái)促進(jìn)其溶解。

2.      本品并非商業(yè)化的臨床藥物，僅用作科研用途，不得用作臨床診斷或治療，不得用于食品或藥品，絕對禁止用在人身上。  

3.      為了您的安全和健康，請穿實(shí)驗服并戴一次性手套操作。

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