SBFI AM ( Na+ Indicator) 鈉離子指示探針

【溫馨提示】：見(jiàn)我司整理的鈉離子載體/探針（Sodium ionophores/indicators）產(chǎn)品專(zhuān)題。

【務(wù)必注意】：初次使用離子探針的用戶(hù)，強烈建議配合：Pluronic F-127, Cell Culture Tested 細胞培養級（NBS2009-1g）一起使用，以提高探針的水溶性和胞內加載性。

產(chǎn)品簡(jiǎn)介：

SBFI，英文全名Sodium-binding Benzofuran Isophthalate，一種Na+選擇性熒光指示劑，可用來(lái)預測純化線(xiàn)粒體Na+梯度，檢測胞內Na+水平，測定細胞Na+外流，以及與其他熒光指示劑聯(lián)合使用以分析Na+與Ca2+和Mg2+濃度、胞內pH和膜電位變化的相關(guān)性。雖然SBFI對Na+的選擇能力弱于Ca2+指示劑比如Fura-2，但在其他單價(jià)陽(yáng)離子存在體系，SBFI足以檢測Na+的生理濃度。結合離子后的SBFI光譜反應可通過(guò)激發(fā)光比率測定來(lái)判定，其能與用相同光濾片和儀器檢測的探針Fura-2共同使用。

當體系內含生理濃度的K+/Na+（~135mM），SBFI對Na+的解離常數（Kd）為11.3mM；而不存在K+體系，對Na+的Kd為3.8mM。SBFI對Na+的選擇性比K+約強18倍。

本品為乙酰氧基甲基酯（Acetoxymethyl ester, AM ester）形式的SBFI，CAS NO：129423-53-6，具有細胞膜滲透性，只需簡(jiǎn)單孵育即可進(jìn)入細胞，常用加載濃度范圍5-10μM，加載時(shí)間40min-4h，根據具體的實(shí)驗要求和細胞類(lèi)型來(lái)調整。

產(chǎn)品特性：

1）化學(xué)名：4,4’-[1,4,10-trioxa-7,13-diazacyclopentadecane-7,13-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-1,3- benzenedicarboxylic acid 1,1’,3,3’-tetrakis[(acetyloxy)methyl] ester

2）同義名：Sodium-binding Benzofuran Isophthalate Acetoxymethyl ester

3）CAS NO：129423-53-6

4）分子式：C56H58N2O23

5）分子量：1127.07

6）純度：＞90%（HPLC） 

7）Ex/Em：340,380/500 nm

8）外觀(guān)：淺黃至暗黃至暗橙固體或油狀

9）溶解性：溶于DMSO（10mM）

保存條件: 

-20℃避光干燥穩定凍存2年。

注意事項：

1.      SBFI AM易受潮，粉末需要干燥保存；需用無(wú)水DMSO溶解，配制儲存液（如10mM），置于-20℃干燥避光，小量分裝避免反復凍融，至少3個(gè)月穩定。

2.      由于SBFI AM水溶性較差，可在實(shí)驗前與等體積Pluronic F-127（25% w/v）混合，以提高探針加載效率。

3.      為了您的安全和健康，請穿實(shí)驗服并戴一次性手套操作。

文獻引用： 

[1] Tong W et al. Phthalocyanine functionalized poly(glycidyl methacrylate) nano-assemblies for photodynamic inactivation of bacteria. Biomater. Sci., 2019,7, 1905-1918

[2] Li R et al. Biofilm inhibition and mode of action of epigallocatechin gallate against Vibrio mimicus. Food Control, Volume 113, July 2020, 107148
Then PBFI probe was added and incubated at 37 °C for 90 min.The cells were washed, collected and resuspended with PBS buffer. Aliquots (100 μL) of bacterial suspension were transferred to a Corning 96 well black plate, and 100 μL of various concentrations of EGCG were dispensed in the microtiter plate wells.

[3] Liu Y, Zhen W, Wang Y, Song S, Zhang H. Na2S2O8 Nanoparticles Trigger Antitumor Immunotherapy through Reactive Oxygen Species Storm and Surge of Tumor Osmolarity. J Am Chem Soc. 2020 Dec 30;142(52):21751-21757. doi: 10.1021/jacs.0c09482. Epub 2020 Dec 18. PMID: 33337859.
4T1 cells were inoculated into glass bottom culture dishes for 24 h. Then, adding PNSO NPs medium solution (80 μg/mL) to continue co-culture for 4 h. The treated 4T1 cells were further incubated with 10μM Na+ indicator SBFI AM in 0.04% Pluronic F-127 and the fluorescence signal was measured by CLSM.

[4] Liang Z, Yang Y, Yu G, et al. Engineering aluminum hydroxyphosphate nanoparticles with well-controlled surface property to enhance humoral immune responses as vaccine adjuvants. Biomaterials. 2021 Jun;275:120960. DOI: 10.1016/j.biomaterials.2021.120960.
[5] BMDMs were treated with AAHPs (250 μg/mL) in the presence of LPS at 500 ng/mL for 5 h.Then PBFI AM was added to the cells at the concentration of 10 μM, and cells were incubated at 37 °C for 1h. Triton X-100 (0.2%) treated cells were used as controls. The fluorescence of PBFI AM was measured at the Ex/Em of 340/615 nm. The data were expressed as relative fluorescence intensity (RFI) defined as the fluorescence intensity of AAHPs-treated BMDMs normalized to the intensity of control cells.

[6] Jia Y, Yang B, Shi J, Fang D, Wang Z, Liu Y. Melatonin prevents conjugative transfer of plasmid-mediated antibiotic resistance genes by disrupting proton motive force. Pharmacol Res. 2022 Jan;175:105978. doi: 10.1016/j.phrs.2021.105978. Epub 2021 Nov 21. PMID: 34813930.
As for the detection of intracellular K + concentration, the PBFI-AM (K + indicator) fluorescence dye labeled cells (10 μM) in the presence of…..

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